The building has 5,110 square metres of lettable space, with anchor tenants in IDP Education and Bankwest taking up 739sqm collectively.
The four-storey property was also formerly Bankwest's administrative centre.
Colliers International director of investment sales Ian Mickle said the remainder of the building would be offered with vacant possession.
“This is a quality office building with generous and flexible floor plates, and the offer of vacant possession makes this a very appealing asset,” Mr Mickle said.
“Buyers can enjoy leasing income from the ground floor tenancies while having the freedom to fit out and use the upper levels to suit their needs.”
Mr Mickle said the building also offered considerable development potential, with its plot ratio recently having been increased to 4:1.
With the new plot ration, the site could accommodate a building of up to 6,600sqm, Mr Mi9ckle said.
Offers for 100 James Street close on June 13.